Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta.
نویسندگان
چکیده
Trametes hirsuta and a purified laccase from this organism were able to degrade triarylmethane, indigoid, azo, and anthraquinonic dyes. Initial decolorization velocities depended on the substituents on the phenolic rings of the dyes. Immobilization of the T. hirsuta laccase on alumina enhanced the thermal stabilities of the enzyme and its tolerance against some enzyme inhibitors, such as halides, copper chelators, and dyeing additives. The laccase lost 50% of its activity at 50 mM NaCl while the 50% inhibitory concentration (IC(50)) of the immobilized enzyme was 85 mM. Treatment of dyes with the immobilized laccase reduced their toxicities (based on the oxygen consumption rate of Pseudomonas putida) by up to 80% (anthraquinonic dyes). Textile effluents decolorized with T. hirsuta or the laccase were used for dyeing. Metabolites and/or enzyme protein strongly interacted with the dyeing process indicated by lower staining levels (K/S) values than obtained with a blank using water. However, when the effluents were decolorized with immobilized laccase, they could be used for dyeing and acceptable color differences (DeltaE*) below 1.1 were measured for most dyes.
منابع مشابه
Characterization and Decolorization of Dye and Textile Effluent by Laccase from Pleurotus Florida- a White-rot Fungi
The textile industries by for the most passionate user of synthetic dyes are in need of ecoefficient solutions for its coloured effluents. The decolorization and detoxification potential of white rot fungi can be harnessed. Laccase has been utilized in biological treatment of effluents containing chemical dyes. The present study is planned to study the screening and chrecterization of laccase f...
متن کاملخالصسازی آنزیم لاکاز قارچ Trametes و تعیین خصوصیات فیزیکوشیمیایی لاکاز نوترکیب: یک مطالعه آزمایشگاهی
Background and Objectives: Laccase is the most abundant member of protein family that catalyzes the oxidation of substituted phenols. Laccases are used as biocatalysts for decolorization and bleaching in dye industries, detoxification in environment, and juice clarification in food industries. The present study aimed at producing recombinant laccase, purifying with high yield and fold, and char...
متن کاملBiosorption of Textile Dyes and Effluents by Pleurotusflorida and Trametes Hirsutawith Evaluation of Their Laccase Activity
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متن کاملDecolorization and biodegradation of reactive sulfonated azo dyes by a newly isolated Brevibacterium sp. strain VN-15
Azo dyes constitute the largest and most versatile class of synthetic dyes used in the textile, pharmaceutical, food and cosmetics industries and represent major components in wastewater from these industrial dying processes. Biological decolorization of azo dyes occurs efficiently under low oxygen to anaerobic conditions. However, this process results in the formation of toxic and carcinogenic...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 66 8 شماره
صفحات -
تاریخ انتشار 2000